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cell lysate mixture  (Proteintech)


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    Proteintech cell lysate mixture
    Cell Lysate Mixture, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lysate+mixture/CEL+Antibody/pmc10873635__pnas__2318024121__sapp-110-5-24
    Average 96 stars, based on 2373 article reviews
    cell lysate mixture - by Bioz Stars, 2026-09
    96/100 stars

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    A) TALE trimer with three repeats. Each repeat contained 34 AAs with one of the four different RVDs. Correspondingly, the coding DNA sequence contained 102 bp. B) Schematic of PCR and iUSER treatment to generate 3’ overhangs. C) Strategy for three steps of TALE vector assembly by iUSER cloning.

    Journal: PLOS ONE

    Article Title: Improved USER cloning for TALE assembly and its application to base editing

    doi: 10.1371/journal.pone.0289509

    Figure Lengend Snippet: A) TALE trimer with three repeats. Each repeat contained 34 AAs with one of the four different RVDs. Correspondingly, the coding DNA sequence contained 102 bp. B) Schematic of PCR and iUSER treatment to generate 3’ overhangs. C) Strategy for three steps of TALE vector assembly by iUSER cloning.

    Article Snippet: The target sequence was amplified from the cell lysate by PCR (2× PhantaMax Master mix, Vazyme) with specific primers.

    Techniques: Sequencing, Plasmid Preparation, Clone Assay